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. 1976 Sep 1;157(3):661-6.
doi: 10.1042/bj1570661.

Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with triton X-100

P R Dunkley, H Holmes, R Rodnight

Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with triton X-100

P R Dunkley et al. Biochem J. .

Abstract

Synaptic-membrane fragments from ox cerebral cortex contain basal and cyclic AMP-stimulated protein kinase activity catalysing the phosphorylation of endogenous substrates. Extraction of membrane fragments with Triton X-100 solubilized less than 20% of the kinase activity and left the major part of the endogenous substrates in the insoluble fraction.

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References

    1. Metabolism. 1975 Mar;24(3):331-41 - PubMed
    1. J Biol Chem. 1975 Dec 10;250(23):9044-52 - PubMed
    1. Biochim Biophys Acta. 1976 Apr 16;433(1):223-7 - PubMed
    1. Biochim Biophys Acta. 1971 Dec 3;249(2):406-18 - PubMed
    1. Brain Res. 1973 Nov 9;62(1):135-53 - PubMed