Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Sep 1;157(3):661-6.
doi: 10.1042/bj1570661.

Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with triton X-100

P R Dunkley, H Holmes, R Rodnight

Phosphorylation of synaptic-membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with triton X-100

P R Dunkley et al. Biochem J. .

Abstract

Synaptic-membrane fragments from ox cerebral cortex contain basal and cyclic AMP-stimulated protein kinase activity catalysing the phosphorylation of endogenous substrates. Extraction of membrane fragments with Triton X-100 solubilized less than 20% of the kinase activity and left the major part of the endogenous substrates in the insoluble fraction.

PubMed Disclaimer

References

    1. Metabolism. 1975 Mar;24(3):331-41 - PubMed
    1. J Biol Chem. 1975 Dec 10;250(23):9044-52 - PubMed
    1. Biochim Biophys Acta. 1976 Apr 16;433(1):223-7 - PubMed
    1. Biochim Biophys Acta. 1971 Dec 3;249(2):406-18 - PubMed
    1. Brain Res. 1973 Nov 9;62(1):135-53 - PubMed