Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Jul 1;64(Pt 7):645-7.
doi: 10.1107/S1744309108016394. Epub 2008 Jun 28.

Crystallization, X-ray diffraction analysis and preliminary structure determination of the polygalacturonase PehA from Agrobacterium vitis

Paul B Vordtriede  1 , Marilyn D Yoder
Affiliations

Crystallization, X-ray diffraction analysis and preliminary structure determination of the polygalacturonase PehA from Agrobacterium vitis

Paul B Vordtriede et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Polygalacturonases are pectate-degrading enzymes that belong to glycoside hydrolase family 28 and hydrolyze the alpha-1,4 glycosidic bond between neighboring galacturonasyl residues of the homogalacturonan substrate. The acidic polygalacturonase PehA from Agrobacterium vitis was overexpressed in Escherichia coli, where it accumulated in the periplasmic fraction. It was purified to homogeneity via a two-step chromatography procedure and crystallized using the hanging-drop vapour-diffusion technique. PehA crystals belonged to space group P2(1), with unit-cell parameters a = 52.387, b = 62.738, c = 149.165 A, beta = 89.98 degrees . Crystals diffracted to 1.59 A resolution and contained two molecules per asymmetric unit. An initial structure determination by molecular replacement indicated a right-handed parallel beta-helix fold.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Crystals of PehA. Typical crystals have dimensions of 0.1 × 0.1 × 1.0 mm.
See this image and copyright information in PMC

References

    1. Abbott, D. W. & Boraston, A. B. (2007). J. Mol. Biol.368, 1215–1222. - PubMed
    1. Brencic, A. & Winans, S. C. (2005). Microbiol. Mol. Biol. Rev.69, 155–194. - PMC - PubMed
    1. Burr, T. J., Katz, B. H. & Bishop, A. L. (1987). Plant Dis.71, 617–620.
    1. Cho, S. W., Lee, S. & Shin, W. (2001). J. Mol. Biol.311, 863–878. - PubMed
    1. D’Ovidio, R., Mattei, B., Roberti, S. & Bellincampi, D. (2004). Biochim. Biophys. Acta, 1696, 237–244. - PubMed

Publication types

MeSH terms