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. 2008 Oct;46(10):3514-6.
doi: 10.1128/JCM.00966-08. Epub 2008 Jul 16.

Genetic lineages of community-associated methicillin-resistant Staphylococcus aureus in Kuwait hospitals

E E Udo  1 , F G O'Brien, N Al-Sweih, Bobby Noronha, B Matthew, W B Grubb
Affiliations

Genetic lineages of community-associated methicillin-resistant Staphylococcus aureus in Kuwait hospitals

E E Udo et al. J Clin Microbiol. 2008 Oct.

Abstract

Twenty-six community-associated methicillin-resistant Staphylococcus aureus (CAMSRA) isolates were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and screened for accessory gene regulator (agr), capsular polysaccharide (cap), and Panton-Valentine leucocidin (PVL) genes. They exhibited five PFGE patterns (types A to E). The majority were PFGE type A (12 isolates) or type B (8 isolates). MLST showed that PFGE type A isolates belonged to sequence type 80 (ST80), while the PFGE type B isolates were ST30. The ST80 and ST30 clones contained agr allotype 3, cap type 8, and PVL. The results showed that two internationally recognized CAMRSA clones are dominant in Kuwait hospitals.

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Figures

FIG. 1.
FIG. 1.
PFGE of representative CAMRSA isolates. Lane 1, PFGE type A, ST80; lane 2, PFGE type A1, ST80; lane 3, PFGE type A2, ST80; lane 4, PFGE type B, ST30; lane 5, PFGE type B1, ST30; lane 6, PFGE type C, ST8; lane 7, PFGE type C1, ST6; lane 8, PFGE type C2, ST8; lane 9, PFGE type D, ST5; lane 10, PFGE type E, ST361; lane M, molecular size marker.
See this image and copyright information in PMC

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