Analytical investigation: assay of D-lactate in diabetic plasma and urine

Abstract

Objective: In this study we developed a 96-micro plate enzymatic assay for D-lactate in plasma and urine.

Methods: D-lactate was assayed enzymatically with a UV-spectrophotometer in plasma from 38 and in urine from 37 diabetics and from 42 non-diabetic controls.

Results: The within-run coefficients of variations (CV) were 2.6% for plasma and 5.7% for urine. The between run CVs were 6.8% for plasma and 6.7% for urine. The mean recovery with standard deviation (S.D.) was 107.4+/-7.3% for plasma and 100.1%+/-6.7% for urine. The plasma D-lactate in diabetics were (mean+/-S.D.) 39.6+/-23.7 microM. We found significant difference between the urinary d-lactate in controls and diabetics (18.2+/-12.0 vs. 35.9+/-24.2 microM/mM creatinine, p<0.0001).

Conclusions: The assay proved reliable with acceptable precision and recovery. Results suggest that diabetics have elevated urinary and plasma D-lactate as compared to controls.