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. 2007 Oct;144(4):515-9.
doi: 10.1007/s10517-007-0365-7.

Epitope mapping of human Fas using peptide phage display

S G Abbasova  1 , Zh M Shcheprova, A G Laman, A O Shepelyakovskaya, L K Baidakova, I L Rodionov, N E Kushlinskii
Affiliations

Epitope mapping of human Fas using peptide phage display

S G Abbasova et al. Bull Exp Biol Med. 2007 Oct.

Abstract

Sandwich EIA for measurement of soluble Fas was developed on the basis of SA-7 and SA-8 monoclonal antibodies to full-length human Fas. The threshold sensitivity of the test system is 0.3 ng/ml. Several isoforms of soluble Fas were identified. The structure of SA-7 and SA-8 antibody epitopes was determined using the peptide phage library. It was shown that SA-7 antibody epitope is determined by amino acid residues 129-134 (CKPNFF), while SA-8 antibody epitope is determined by amino acid residues 94-99 (KAHFSS) of full-length Fas. Hence, sandwich EIA on the basis of SA-7 and SA-8 monoclonal antibodies for detection of soluble Fas in human serum is to detect the following Fas isoforms: FasExo6Del, FasExo4Del, FasExo4,6Del, FasExo4.7Del, and FasExo8Del.

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