Chlorophyll levels in the pigment-binding proteins of photosystem II. A study based on the chlorophyll to cytochrome ratio in different photosystem II preparations

Abstract

The chlorophyll levels in pigment proteins of photosystem II were investigated by using photosystem II preparations with different levels of complexity. Based on the assumption that there is 1 cytochrome b559 per reaction centre it has been found that oxygen-evolving complexes containing CP26 and CP29 bind 42 chlorophyll molecules. When CP26 and CP29 are stripped away, the resulting PSII cores bind 30 chlorophyll molecules while CP43-less cores bind approximately 18 chlorophylls. It is therefore concluded that CP47 and CP43 bind 9-12 molecules of chlorophyll a and the D1/D2 complex binds 6 chlorophylls. Taken together CP26 and CP29 bind about 12 chlorophyll molecules.