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. 1991 Jun 15;102(1):123-7.
doi: 10.1016/0378-1119(91)90550-u.

Sequence and expression in Escherichia coli of a Mycoplasma hominis gene encoding elongation factor Tu

E Lüneberg  1 , V Kamla, U Hadding, M Frosch
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Sequence and expression in Escherichia coli of a Mycoplasma hominis gene encoding elongation factor Tu

E Lüneberg et al. Gene. .

Abstract

We describe the molecular cloning and the complete nucleotide (nt) sequence of a Mycoplasma hominis gene common to a broad range of Mycoplasma species, as defined by hybridization analysis with the cloned gene. Production of M. hominis protein in Escherichia coli was assayed by use of a monoclonal antibody. The nt sequence analysis revealed a 1194-bp open reading frame that could encode a 43 516-Da protein. Computer-aided sequence comparison indicated that the gene codes for elongation factor Tu.

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