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. 2007 Jun;8(4):234-51.
doi: 10.2174/138920207781386960.

Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes

Sa Deepak  1 , Kr Kottapalli, R Rakwal, G Oros, Ks Rangappa, H Iwahashi, Y Masuo, Gk Agrawal
Affiliations

Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes

Sa Deepak et al. Curr Genomics. 2007 Jun.

Abstract

Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21(st) century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.

Keywords: Real-time PCR; applications; detection; disease; microorganisms; pathogen; plant-microbe interaction; quantification.

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Sampling procedures are of great importance towards the validation of analytical methods for analysis.
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