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. 1991 Jul;87(3):261-5.
doi: 10.1007/BF00200901.

Non-isotopic detection of chromosome 1 in human meiosis and demonstration of disomic sperm nuclei

M Guttenbach  1 , M Schmid
Affiliations

Non-isotopic detection of chromosome 1 in human meiosis and demonstration of disomic sperm nuclei

M Guttenbach et al. Hum Genet. 1991 Jul.

Abstract

Nonradioactive in situ hybridization with the biotin-labeled chromosome 1-specific probe pUC1.77 was performed on human mitotic and meiotic chromosomes, and on sperm nuclei. The streptavidine-horseradish-peroxidase and diaminobenzidine detection system demonstrated heteromorphisms in the 1q12 heterochromatic region, not only in mitotic cells but also in mature sperm heads. The localization of chromosome 1 could be traced through all meiotic stages and in the sperm nuclei. The frequency of chromosome 1 disomy in human sperm, as indicated by two distinct hybridization signals, was calculated to be 0.41%.

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