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. 2008 Aug 11;9(12):1902-5.
doi: 10.1002/cbic.200800183.

TokenRNA: a new type of sequence-specific, label-free fluorescent biosensor for folded RNA molecules

Kirill A Afonin  1 , Evgeny O Danilov, Irina V Novikova, Neocles B Leontis
Affiliations

TokenRNA: a new type of sequence-specific, label-free fluorescent biosensor for folded RNA molecules

Kirill A Afonin et al. Chembiochem. .
No abstract available

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Figures

Figure 1
Figure 1
Annotation of the MG aptamer 3D structure using the Leontis-Westhof basepair nomenclature.[18] Non-Watson–Crick base-pairs are annotated to show interacting edges: Watson–Crick (WC) Edge (circles), Hoogsteen Edge (squares), or the Sugar Edge (triangles). Open symbols indicate trans pairs and solid symbols, cis pairs. Boxes are placed around bases involved in more than one basepair interaction.19 The bases of the aptamer are shown in green.
Figure 2
Figure 2
MG aptamer enhances the fluorescence of MG upon the assembly of 36Mtoken/36Mtarget recognition complex. Emission spectra of free MG (-----), MG in the presence of aptamer-containing 36Mtoken only (····), and MG in the presence of the recognition complex 36Mtoken/36Mtarget containing the stabilized MG aptamer site (–·–·–).
Figure 3
Figure 3
Schematic representation of the sequence specificity of target RNA recognition and supporting emission spectra. A) Single point mutation (G11→C) in the target, 36Mtarget(a), blocks recognition by 36Mtoken. B) Compensatory substitution (C6→G) in 36Mtoken(a) supports recognition of 36Mtarget(a) and turns on Malachite Green (MG) emission signal.
Scheme 1
Scheme 1
Schematic representation of the assembly of 36Mtoken and 36Mtarget to form the recognition complex, stabilizing the Malachite Green (MG) aptamer site (see Figure 1).
See this image and copyright information in PMC

References

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