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. 2008 Jul;20(7):1736-7.
doi: 10.1105/tpc.108.061143. Epub 2008 Jul 29.

Eleven golden rules of quantitative RT-PCR

Michael K Udvardi, Tomasz Czechowski, Wolf-Rüdiger Scheible

Eleven golden rules of quantitative RT-PCR

Michael K Udvardi et al. Plant Cell. 2008 Jul.
No abstract available

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Comment in

  • Refining our standards.
    Martin C. Martin C. Plant Cell. 2008 Jul;20(7):1727. doi: 10.1105/tpc.108.200780. Epub 2008 Jul 29. Plant Cell. 2008. PMID: 18664610 Free PMC article. No abstract available.

References

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    1. Czechowski, T., Stitt, M., Altmann, T., Udvardi, M.K., and Scheible, W.R. (2005). Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol. 139 5–17. - PMC - PubMed
    1. Pfaffl, M.W., Tichopad, A., Prgomet, C., and Neuvians, T.P. (2004). Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26 509–551. - PubMed
    1. Ramakers, C., Ruijter, J.M., Deprez, R.H., and Moorman, A.F. (2003). Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett. 339 62–66. - PubMed
    1. Ståhlberg, A., Håkansson, J., Xian, X., Semb, H., and Kubista, M. (2004). Properties of the reverse transcription reaction in mRNA quantification. Clin. Chem. 50 509–515. - PubMed

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