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. 1977 Jul 5;16(2):141-8.
doi: 10.1007/BF01732055.

A primer independent activity of rabbit muscle phosphorylase b

J Tandecarz, N Lavintman, C E Cardini

A primer independent activity of rabbit muscle phosphorylase b

J Tandecarz et al. Mol Cell Biochem. .

Abstract

Rabbit muscle phosphorylase b was found to be capable of forming protein bound alpha-1,4 glucosyl chains upon incubation of the enzyme with appropriate concentrations of glucose-1-phosphate with no primer addition (unprimed synthesis). This activity would only be present in a small fraction of the total muscle phosphorylase b activity, as judged from the high concentrations of enzyme which are required to demonstrate the occurrence of unprimed synthesis. Polyacrylamide gel electrophoresis shows the presence of a phosphorylase isoenzyme capable of accepting glucosyl moieties, giving rise to a glucosylated protein enzymatically active in the chain lengthening of its own glucan.

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References

    1. Proc Natl Acad Sci U S A. 1961 Apr 15;47:469-78 - PubMed
    1. Plant Physiol. 1971 Dec;48(6):765-9 - PubMed
    1. Ann N Y Acad Sci. 1964 Dec 28;121:404-27 - PubMed
    1. Anal Biochem. 1962 Jul;4:17-23 - PubMed
    1. Biochem Biophys Res Commun. 1972 Feb 16;46(3):1206-12 - PubMed