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. 2008 Oct;43(2):219-22.
doi: 10.1016/j.jcv.2008.06.007.

Multiplex MassTag-PCR for respiratory pathogens in pediatric nasopharyngeal washes negative by conventional diagnostic testing shows a high prevalence of viruses belonging to a newly recognized rhinovirus clade

Samuel R Dominguez  1 , Thomas Briese, Gustavo Palacios, Jeffrey Hui, Joseph Villari, Vishal Kapoor, Rafal Tokarz, Mary P Glodé, Marsha S Anderson, Christine C Robinson, Kathryn V Holmes, W Ian Lipkin
Affiliations

Multiplex MassTag-PCR for respiratory pathogens in pediatric nasopharyngeal washes negative by conventional diagnostic testing shows a high prevalence of viruses belonging to a newly recognized rhinovirus clade

Samuel R Dominguez et al. J Clin Virol. 2008 Oct.

Abstract

Background: Respiratory infections are the most common infectious diseases in humans worldwide and are a leading cause of death in children less than 5 years of age.

Objectives: Identify candidate pathogens in pediatric patients with unexplained respiratory disease.

Study design: Forty-four nasopharyngeal washes collected during the 2004-2005 winter season from pediatric patients with respiratory illnesses that tested negative for 7 common respiratory pathogens by culture and direct immunofluorescence assays were analyzed by MassTag-PCR. To distinguish human enteroviruses (HEV) and rhinoviruses (HRV), samples positive for picornaviruses were further characterized by sequence analysis.

Results: Candidate pathogens were detected by MassTag PCR in 27 of the 44 (61%) specimens that previously were rated negative. Sixteen of these 27 specimens (59%) contained picornaviruses; of these 9 (57%) contained RNA of a recently discovered clade of rhinoviruses. Bocaviruses were detected in three patients by RT-PCR.

Conclusions: Our study confirms that multiplex MassTag-PCR enhances the detection of pathogens in clinical specimens, and shows that previously unrecognized rhinoviruses, that potentially form a species HRV-C, may cause a significant amount of pediatric respiratory disease.

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References

    1. WHO, 2003. The World Health Report 2003: shaping the future. World Health Organization.
    1. Klig J.E., Shah N.B. Office pediatrics: current issues in lower respiratory infections in children. Curr Opin Pediatr. 2005;17:111–118. - PubMed
    1. Klig J.E. Current challenges in lower respiratory infections in children. Curr Opin Pediatr. 2004;16:107–112. - PubMed
    1. Fendrick A.M., Monto A.S., Nightengale B., Sarnes M. The economic burden of non-influenza-related viral respiratory tract infection in the United States. Arch Intern Med. 2003;163:487–494. - PubMed
    1. Griffin M.R., Walker F.J., Iwane M.K., Weinberg G.A., Staat M.A., Erdman D.D. Epidemiology of respiratory infections in young children: insights from the new vaccine surveillance network. Pediatr Infect Dis J. 2004;23:S188–S192. - PubMed

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