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. 1991 Apr;10(4):426, 428.

S1 nuclease transcript mapping using sequenase-derived single-stranded probes

A D Sharrocks  1 , D P Hornby
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  • PMID: 1867849

S1 nuclease transcript mapping using sequenase-derived single-stranded probes

A D Sharrocks et al. Biotechniques. 1991 Apr.

Abstract

We report here a simple method for the production of high specific activity single-stranded DNA probes for S1 nuclease transcript mapping. Plasmid DNA is used as in vitro DNA templates for probe production by primer extension using Sequenase. This allows the production of long single-stranded probes without the need to subclone restriction fragments into M13 vectors. The mapping of the 5' ends of the phage P1 mod gene transcripts is shown as an example.

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