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. 1991 Apr;10(4):474, 476-7.

Rapid identification of specific genes in E. coli by hybridization to membranes containing the ordered set of phage clones

A Noda  1 , J B Courtright, P F Denor, G Webb, Y Kohara, A Ishihama
Affiliations
  • PMID: 1867855

Rapid identification of specific genes in E. coli by hybridization to membranes containing the ordered set of phage clones

A Noda et al. Biotechniques. 1991 Apr.

Abstract

A simplified protocol for the hybridization of labeled oligonucleotides and other radiolabeled DNAs to membranes is described and used for the detection of specific recombinant phage in the ordered miniset collection representing virtually the entire E. coli genome.

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