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Review
. 2008 Sep;130(3):435-45.
doi: 10.1007/s00418-008-0479-7. Epub 2008 Aug 5.

RNA expression analysis from formalin fixed paraffin embedded tissues

Susan M Farragher  1 , Austin Tanney, Richard D Kennedy, D Paul Harkin
Affiliations
Review

RNA expression analysis from formalin fixed paraffin embedded tissues

Susan M Farragher et al. Histochem Cell Biol. 2008 Sep.

Abstract

Formalin fixation and paraffin embedding (FFPE) is the most commonly used method worldwide for tissue storage. This method preserves the tissue integrity but causes extensive damage to nucleic acids stored within the tissue. As methods for measuring gene expression such as RT-PCR and microarray are adopted into clinical practice there is an increasing necessity to access the wealth of information locked in the Formalin fixation and paraffin embedding archives. This paper reviews the progress in this field and discusses the unique opportunities that exist for the application of these techniques in the development of personalized medicine.

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References

    1. J Clin Oncol. 2008 Feb 10;26(5):729-35 - PubMed
    1. Zentralbl Veterinarmed B. 1994 Dec;41(10):654-61 - PubMed
    1. Nucleic Acids Res. 1999 Nov 15;27(22):4436-43 - PubMed
    1. BMC Mol Biol. 2007 Dec 21;8:118 - PubMed
    1. Pathol Int. 1997 Oct;47(10):685-91 - PubMed

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