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. 1991 Sep;10(9):2589-94.
doi: 10.1002/j.1460-2075.1991.tb07800.x.

Flexibility of the DNA enhances promoter affinity of Escherichia coli RNA polymerase

W Werel  1 , P Schickor, H Heumann
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Flexibility of the DNA enhances promoter affinity of Escherichia coli RNA polymerase

W Werel et al. EMBO J. 1991 Sep.

Abstract

Two types of mechanisms are discussed for the formation of active protein-DNA complexes: contacts with specific bases and interaction via specific DNA structures within the cognate DNA. We have studied the effect of a single nucleoside deletion on the interaction of Escherichia coli RNA polymerase with a strong promoter. This study reveals three patterns of interaction which can be attributed to different sites of the promoter, (i) direct base contact with the template strand in the '-35 region' (the 'recognition domain'), (ii) a DNA structure dependent interaction in the '-10 region' (the 'melting domain'), and (iii) an interaction which is based on a defined spatial relationship between the two domains of a promoter, namely the 'recognition domain' and the 'melting domain'.

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References

    1. J Mol Biol. 1987 Jun 20;195(4):795-808 - PubMed
    1. J Mol Biol. 1989 Apr 20;206(4):591-603 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6673-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5469-73 - PubMed
    1. Nucleic Acids Res. 1989 Feb 25;17(4):1783 - PubMed

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