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. 2008 Oct;190(20):6927-30.
doi: 10.1128/JB.00801-08. Epub 2008 Aug 8.

Paerucumarin, a new metabolite produced by the pvc gene cluster from Pseudomonas aeruginosa

Michael F Clarke-Pearson  1 , Sean F Brady
Affiliations

Paerucumarin, a new metabolite produced by the pvc gene cluster from Pseudomonas aeruginosa

Michael F Clarke-Pearson et al. J Bacteriol. 2008 Oct.

Abstract

The pvc gene cluster from Pseudomonas aeruginosa has been linked to the biosynthesis of both the pyoverdine chromophore and pseudoverdine. Our reinvestigation of the role this gene cluster plays in P. aeruginosa secondary metabolite biosynthesis shows that its major product is actually paerucumarin, a novel isonitrile functionalized cumarin.

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Figures

FIG. 1.
FIG. 1.
(a) pvc gene cluster. (b) Proposed biosynthetic scheme for paerucumarin. (c) Proposed biosynthetic scheme for the pyoverdine chromophore (6).
FIG. 2.
FIG. 2.
LC-MS (negative-ion electrospray ionization) traces of the ethyl acetate extracts derived from IPTG-induced and uninduced cultures of P. aeruginosa transformed with pPVCAD are shown.
FIG. 3.
FIG. 3.
LC-MS (negative-ion electrospray ionization) traces of ethyl acetate extracts from cultures of PAK (a), PAO1 (b), and PA14 (c) strains transformed with pPTXR and strain PAK (d) transformed with a vector control. Peaks for paerucumarin (peak 1) and pseudoverdine (peak 2) are marked.
FIG. 4.
FIG. 4.
High-pressure liquid chromatography (UV) traces of the material extracted by solid-phase extraction from cultures of strains PAK (a), PAK (ΔpvcA) (b), and PAK2 (ΔpvdI) (c).
See this image and copyright information in PMC

References

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