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. 2008;47(38):7284-8.
doi: 10.1002/anie.200801810.

Micellar hybrid nanoparticles for simultaneous magnetofluorescent imaging and drug delivery

Ji-Ho Park  1 , Geoffrey von Maltzahn, Erkki Ruoslahti, Sangeeta N Bhatia, Michael J Sailor
Affiliations

Micellar hybrid nanoparticles for simultaneous magnetofluorescent imaging and drug delivery

Ji-Ho Park et al. Angew Chem Int Ed Engl. 2008.
No abstract available

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Figures

Figure 1
Figure 1
Transmission electron microscope images of a) micellar hybrid nanoparticles (MHN) with a mass ratio of 1:5 magnetic nanoparticles (MN): quantum dots (QD) (inset: TEM image of an individual MHN that has been treated with a 1.3% phosphotungstic acid negative stain. The brighter regions are associated with the micellar coating). b–d) magnified images of MHN with a mass ratio of b) 1:1 MN:QD (MHN1); c) 1:3 MN:QD (MHN3); d) 1:5 MN:QD (MHN5). e) micellar magnetic nanoparticles. f) micellar quantum dots (emission λmax = 705 nm). Scale bar in (a) is 100 nm. Scale bar in (b) is 20 nm; images (b–d) and the inset of (a) are displayed at the same magnification. Scale bar in (e) is 20 nm and (f) is displayed at the same magnification. In these formulations the QD have an elongated shape (2:1 aspect) and the MN are spherical.
Figure 2
Figure 2
a) Photoluminescence spectra of micellar quantum dots (MQD, emission λmax = 705 nm), micellar magnetic nanoparticles (MMN) and micellar hybrid nanoparticles (MHN) containing different ratios of MN:QD. The particle samples were excited with 450 nm light. The intensity (I) of each spectrum is normalized by total mass of each particle type. b) Multimodal imaging of MMN and MHN as a function of iron concentration in MRI (upper panel, T2-weighted mode) and NIR fluorescence (lower, in the Cy5.5 fluorescence channel, λex = 680 nm, λobs = 720 nm). c) Relaxivity R2 values of MMN and MHN in the T2-weighted MR images.
Figure 3
Figure 3
a) Intracellular delivery of F3-conjugated micellar hybrid nanoparticles (F3-MHN) into MDA-MB-435 human carcinoma cells. In both panels the F3-MHN or the MHN control particles appear red in the images. 2 h after incubation with the cells, the F3-MHN particles are strongly associated with the cells, while the control nanoparticles (MHN) without the F3 species do not penetrate. b) Multimodal images (NIR fluorescence in Cy5.5 channel and MRI) of the cells in (a) compared with PBS control and with untreated cells. c) Targeted drug delivery of doxorubicin (DOX)-incorporated F3-MHN into MDA-MB-435 human carcinoma cells. The DOX-loaded F3-MHN were incubated with the cells for 2 h. Arrowheads indicate co-localization of DOX and MHN. The inset shows co-localization of some DOX (red) and endosome marker (green) 30 min after incubation with DOX-loaded F3-MHN. Nuclei were stained with DAPI.
Figure 4
Figure 4
a) NIR fluorescence images showing passive accumulation of micellar hybrid nanoparticles containing QD (emitting at 800 nm, MHN(800)) in a mouse bearing MDA-MB-435 tumors. The mouse was imaged pre-injection and 20 h post-injection (injection dose: 10 mg/kg). b) Image table representing multimodal imaging (by MRI and NIR fluorescence) of tumor harvested from the mouse in (a). PBS indicates a control in which a tumor-bearing mouse was injected with phosphate buffered saline. Column headings NIRFI and MRI(T2) indicate near-infrared fluorescence image and T2 values from T2-weighted MRI, respectively.
Scheme 1
Scheme 1
The synthetic procedure used to prepare micellar hybrid nanoparticles that encapsulate magnetic nanoparticles and quantum dots within a single PEG-modified phopholipid micelle.
See this image and copyright information in PMC

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