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. 1976:1:159-62.

Kinetic studies on 15-hydroxyprostaglandin dehydrogenase from human placenta

W Schlegel, R O Greep
  • PMID: 187034

Kinetic studies on 15-hydroxyprostaglandin dehydrogenase from human placenta

W Schlegel et al. Adv Prostaglandin Thromboxane Res. 1976.

Abstract

The enzyme system 15-hydroxyprostaglandin dehydrogenase, which catalyzes the oxidation of the 15-hydroxy group of all naturally occurring prostaglandins, has been purified 1,270-fold by isoelectric focusing. Km values have been determined for prostaglandin E2 and NAD+ and were found to be 1 and 44 muM. The overall forward reaction was V1 = 450 nmol/min. Both the product 15-ketoprostaglandin E2 and the metabolite 13,14-dihydro-15-ketoprostaglandin E2 were noncompetitive inhibitors for the enzyme.

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