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. 2008 Mar;9(2):147-55.
doi: 10.1111/j.1364-3703.2007.00450.x.

Host-specific effect of P1 exchange between two potyviruses

Beatriz Salvador  1 , Pilar Saénz, Emilio Yangüez, Jean Bernard Quiot, Laurence Quiot, María Otilia Delgadillo, Juan Antonio García, Carmen Simón-Mateo
Affiliations

Host-specific effect of P1 exchange between two potyviruses

Beatriz Salvador et al. Mol Plant Pathol. 2008 Mar.

Abstract

The potyviruses Plum pox virus (PPV) and Tobacco vein mottling virus (TVMV) have distinct host ranges and induce different symptoms in their common herbaceous hosts. To test the relevance of the P1 protein in host compatibility and pathogenicity, hybrid viruses were constructed in which the P1 coding sequence of PPV was completely or partially replaced by the corresponding sequences from TVMV. Infections induced by these chimeric viruses revealed that the TVMV P1 and a PPV/TVMV hybrid P1 proteins are functionally equivalent in herbaceous plants to the P1 protein of a PPV isolate adapted to these hosts, in spite of having high sequence divergence. Moreover, the presence of TVMV P1 sequences enhanced the competence of a low-infectivity PPV-D-derived chimera in Nicotiana clevelandii. Conversely, all PPV/TVMV hybrids were unable to infect Prunus persicae, a specific host for PPV, suggesting that TVMV P1 is not functionally competent in this plant. Together, these data highlight the importance of the P1 protein in defining the virus host range.

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Figures

Figure 1
Figure 1
PPV and TVMV P1 protein alignment and PPV/TVMV chimeras. (a) Sequence alignment of PPV and TVMV P1 proteins. Conserved amino acid positions in the sequence alignment are shadowed in black. Open boxes indicate amino acid differences between the PPV isolates. (b) Schematic representation of the different virus constructs used in this work. The pattern assigned to each parental virus is depicted below the constructs. GFP sequences are indicated with an asterisk. The ability of the chimeras to infect Nicotiana species and/or Prunus persicae GF305 is indicated.
Figure 2
Figure 2
Analysis of the infection of PPV‐R/TVMV chimeras in different herbaceous hosts. Symptoms in the inoculated leaves of N. clevelandii at 15 dpi and C. foetidum at 7 dpi and in the upper non‐inoculated leaves of N. clevelandii and N. benthamiana at 15 dpi.
Figure 3
Figure 3
Analysis of NBD‐GFP, NBD‐Tm‐GFP and NBD‐Tc‐GFP infection in N. clevelandii and GF305 peach. (a) Symptoms and GFP expression observed under visible or UV light, respectively, at 21 days post‐inoculation (dpi) for N. clevelandii and at 35 dpi for GF305 peach. (b) Virus accumulation in upper non‐inoculated infected leaves of N. clevelandii (21 dpi) and GF305 peach (35 dpi) plants. Bars represent the average values and standard deviations of four N. clevelandii and eight peach plants.
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