Genome sequence of the Bacteroides fragilis phage ATCC 51477-B1

Abstract

The genome of a fecal pollution indicator phage, Bacteroides fragilis ATCC 51477-B1, was sequenced and consisted of 44,929 bases with a G+C content of 38.7%. Forty-six putative open reading frames were identified and genes were organized into functional clusters for host specificity, lysis, replication and regulation, and packaging and structural proteins.

Figure 1

Transmission electron micrographs of phage ATCC 51477. (A) Magnified view of a single phage (bar = 50 nm). (B) Four additional phage all displaying tail fibers (bar = 200 nm).

Figure 2

Phage ATCC 51477-B1 genome map. The direction and size of 46 putative ORFs are illustrated with arrows. ORFs labeled in green display high similarity to proteins with known function. ORFs labeled in red display high similarity to other putative open reading frames without assigned functions. ORFs labeled in blue have low similarity (< 0.1) to other putative open reading frames. Sequences with high DNA similarity to the phage B40-8 structural proteins are shown in yellow. Putative B. fragilis promoters are shown as inverted orange triangles, the location of repeats are shown as purple boxes, and polymorphisms are indicated with black lines.