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. 2008 Oct;46(10):3479-81.
doi: 10.1128/JCM.01254-08. Epub 2008 Aug 20.

Deployable laboratory response to emergence of melioidosis in central Sri Lanka

Timothy J J Inglis  1 , Adam Merritt, Joanne Montgomery, Indika Jayasinghe, Vasanthi Thevanesam, Russell McInnes
Affiliations

Deployable laboratory response to emergence of melioidosis in central Sri Lanka

Timothy J J Inglis et al. J Clin Microbiol. 2008 Oct.

Abstract

A portable molecular diagnostic laboratory was used to provide molecular confirmation of suspected melioidosis cases seen at Peradeniya Hospital, central Sri Lanka. Soil supernatants from rice field and rubber plantation samples also produced PCR-positive results. These procedures could be used for melioidosis field work in other remote locations.

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Figures

FIG. 1.
FIG. 1.
Lane L contains the DNA ladder. Lanes 1 to 10 contain DNA extracts from bacteria isolated from patient samples at undiluted and 10−1 dilution. Lane 11 contains a positive control at a dilution of 10−6, and lane 12 contains the nontemplate control (ultrapure water [UPW]). The product demonstrated is an lpxO PCR amplicon of 110 bp in length. pos, positive.
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