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. 2008 Oct;287(2):185-91.
doi: 10.1111/j.1574-6968.2008.01308.x. Epub 2008 Aug 21.

The phage K lytic enzyme LysK and lysostaphin act synergistically to kill MRSA

Stephen C Becker  1 , Juli Foster-Frey, David M Donovan
Affiliations

The phage K lytic enzyme LysK and lysostaphin act synergistically to kill MRSA

Stephen C Becker et al. FEMS Microbiol Lett. 2008 Oct.

Abstract

LysK is the endolysin from the staphylococcal bacteriophage K, and can digest the cell wall of many staphylococci. Lysostaphin is a bacteriocin secreted by Staphylococcus simulans to kill Staphylococcus aureus. Both LysK and lysostaphin have been shown to lyse methicillin-resistant S. aureus (MRSA). This study describes optimal reaction conditions for the recombinant His-tagged LysK protein (pH range pH 6-10, and 0.3-0.5 M NaCl), and C-His-LysK MIC (32.85+/-4.87 mug mL(-1)). LysK and lysostaphin demonstrate antimicrobial synergy by the checkerboard assay.

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