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. 2008 Oct;74(20):6457-60.
doi: 10.1128/AEM.00895-08. Epub 2008 Aug 22.

Isolation and identification of bifidobacteriaceae from human saliva

David Beighton  1 , Steven C Gilbert, Douglas Clark, Maria Mantzourani, Mustafa Al-Haboubi, Farida Ali, Elizabeth Ransome, Nicholas Hodson, Michael Fenlon, Liana Zoitopoulos, Jennifer Gallagher
Affiliations

Isolation and identification of bifidobacteriaceae from human saliva

David Beighton et al. Appl Environ Microbiol. 2008 Oct.

Abstract

Bifidobacteriaceae were isolated from saliva and infected dentine by using a mupirocin-based selective medium. Of the saliva samples, 94% harbored bifids. The mean concentration (+/- the standard error) was 4.46 (+/-0.12) log(10)(CFU per ml + 1), and the predominant isolates were Bifidobacterium dentium, B. longum, Scardovia inopinata, Parascardovia denticolens, and Alloscardovia omnicolens.

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Figures

FIG. 1.
FIG. 1.
Cumulative proportions of subjects (n = 192) colonized by bifids and caries-associated microorganisms.
FIG. 2.
FIG. 2.
(A) Comparison of representative independent B. dentium isolates examined by REP-PCR; (B) demonstration of different REP-PCR patterns among B. dentium isolates from the same root caries lesion specimen. Lanes 1, 8, and 14 (counting from the left) in panel A and lanes 1 and 8 (counting from the left) in panel B are molecular weight markers.
FIG. 3.
FIG. 3.
Comparison of independent B. longum isolates examined by REP-PCR and BOX1AR PCR. Lanes 1, 7, and 14 (counting from the left) in both images are molecular weight markers. The isolates (13-4, Mu55-1, Mu57-1, Mu67-1, Mu79-1, Mu77-1, Mu91-2, Mu86-6, Mu92-1, Mu93-1, Mu100-6, and Mu38-2) are in the same order on each gel.
See this image and copyright information in PMC

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