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. 2008 Nov;52(11):4159-62.
doi: 10.1128/AAC.01633-07. Epub 2008 Aug 25.

Plasmid-mediated quinolone resistance determinants qnrA, qnrB, and qnrS among clinical isolates of Enterobacteriaceae in a Korean hospital

Migma Dorji Tamang  1 , Sung Yong Seol, Jae-Young Oh, Hee Young Kang, Je Chul Lee, Yoo Chul Lee, Dong Taek Cho, Jungmin Kim
Affiliations

Plasmid-mediated quinolone resistance determinants qnrA, qnrB, and qnrS among clinical isolates of Enterobacteriaceae in a Korean hospital

Migma Dorji Tamang et al. Antimicrob Agents Chemother. 2008 Nov.

Abstract

Screening of 368 consecutive nonreplicate clinical isolates of Enterobacteriaceae resistant to nalidixic acid and at least one extended-spectrum beta-lactam revealed the presence of qnrA, qnrB, and qnrS determinants, and identified novel qnrB variants, in Citrobacter freundii isolates. This study also revealed, for the first time, the linkage of qnrB, armA, and extended-spectrum and/or AmpC-type beta-lactamase genes on large conjugative plasmids.

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Figures

FIG. 1.
FIG. 1.
Diagram showing the qnrB subtyping approach by RFLP of the 469-bp qnrB PCR product using restriction enzymes MvaI (Fermentas Inc., Hanover, MD), BglII (New England Biolabs, Beverly, MA), HindIII (Roche Diagnostics GmbH, Mannheim, Germany), and FokI (New England Biolabs, Beverly, MA).
FIG. 2.
FIG. 2.
CLUSTAL W alignment of deduced amino acid sequences of the novel qnrB variants described in this study: QnrB13 (GenBank accession no. ABX72042), QnrB14 (ABX72044), and QnrB15 (ABX72227). Asterisks represent amino acid residues identical to those in the QnrB13 sequence.
FIG. 3.
FIG. 3.
(A) Plasmid DNAs extracted from transconjugants. (B through E) Southern hybridization with the qnrB4 (B), blaDHA-1 (C), blaSHV-12 (D), or armA (E) probe. Lanes: A, pKT50654; B, pKT50774 (lacking blaSHV-12); C, pKT51151; D, pKT51196 (lacking armA); E, pKT51198; F, pKT51208; G, pKT51295; H, pKT51321 (lacking blaDHA-1); I, pKT51352; M, BAC-Tracker supercoiled marker.
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References

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