doi: 10.1128/aem.57.6.1870-1872.1991.
Cloning of alginate lyase gene (alxM) and expression in Escherichia coli
Affiliations
- PMID: 1872617
- PMCID: PMC183488
- DOI: 10.1128/aem.57.6.1870-1872.1991
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Cloning of alginate lyase gene (alxM) and expression in Escherichia coli
Appl Environ Microbiol.
1991 Jun.
Abstract
The alxM gene encoding a D-mannuronan-specific alginate lyase has been cloned from a marine bacterium isolated as an epiphyte on the brown alga, Sargassum fluitans. Expression of this gene in Escherichia coli provides a source of this enzyme for probing alginate structure and modifying the mannuronan-rich alginate polymers produced by bacterial pathogens.
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