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. 2008 Jun 5;6(2):243-61.
doi: 10.3390/md20080012.

The occurrence of bioactive micromonosporae in aquatic habitats of the Sunshine Coast in Australia

Glen P Eccleston  1 , Peter R Brooks, D Ipek Kurtböke
Affiliations

The occurrence of bioactive micromonosporae in aquatic habitats of the Sunshine Coast in Australia

Glen P Eccleston et al. Mar Drugs. .

Abstract

Screening strategies based on the ecological knowledge of antibiotic producing microorganisms and their roles in the natural environment are being increasingly employed in the search for novel antibiotic agents. Micromonosporae are common inhabitants of aquatic habitats and have proved to be a continuing source of novel bioactive compounds including antibacterial and antitumor agents. The ecological distribution and frequency of bioactive micromonosporae in Sunshine Coast region aquatic habitats were studied through a range of selective isolation procedures designed to negatively select against the isolation of unwanted microbial taxa commonly associated with marine environments. It was revealed that bioactive compound producing species of micromonosporae were present in the aquatic habitats of the Sunshine Coast region in Australia.

Keywords: Actinomycetes; Aquatic habitats; Bioactive compounds; Gentamicin; Micromonosporae.

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Figures

Figure 1
Figure 1
Average abundance of gentamicin-resistant actinomycete isolates (cfu) per gram of dried sediment sample.
Figure 2
Figure 2
Composition of sediment derived actinomycetes associated with Sunshine Coast aquatic habitats. Micromonosporae Streptomycetes Other/non-streptomycetes
Figure 3
Figure 3
Light microscopic view of Micromonospora isolate USC-714, indicating single spore structures on a stable substrate mycelium typical of this genus.
Figure 4
Figure 4
Phylogenetic tree of 16S rRNA gene sequences obtained from micromonosporae isolates compared against sequences obtained from public databases. The scale bar represents 10% sequence divergence. Fischerella muscicola was used as an out-group (GenBank accession numbers of reference sequences are included in brackets).
Figure 5
Figure 5
HPLC plot of derivatized gentamicin sulfate standard indicating gentamicin fractions (A, B and C) eluting at 8.4, 10.9 and 14.5 minutes: (a) absorption: 230 nm; (b) fluorescence: 455 nm.
Figure 6
Figure 6
HPLC plot of DSM 43816 crude extract indicating peak eluting at 11.2 minutes: (a) absorption: 230 nm; (b) fluorescence: 455 nm.
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