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. 1991 Apr 19;565(1-2):453-6.
doi: 10.1016/0378-4347(91)80409-6.

Determination of dimethyl sulphide in blood and adipose tissue by headspace gas analysis

K Terazawa  1 , H Kaji, H Akabane, T Takatori
Affiliations

Determination of dimethyl sulphide in blood and adipose tissue by headspace gas analysis

K Terazawa et al. J Chromatogr. .

Abstract

A method for the headspace analysis of dimethyl sulphide in blood and adipose tissue has been established. Blood (0.2 ml) or adipose tissue (0.5 g) with added dimethyl sulphide was sealed in a 10-ml vial using PTFE sheet to prevent escape of dimethyl sulphide from the headspace. Equilibration was performed at 60 degrees C for 4 h, and 20 microliters of gaseous phase sampled from the headspace was subjected to gas chromatography (with flame photometric detection). Calibration curves were prepared for the two samples. Linearity was observed in the range from 5-10 micrograms to 2 mg.

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