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Randomized Controlled Trial
. 2009 Apr;33(2):413-8.
doi: 10.1007/s00264-008-0649-1. Epub 2008 Aug 27.

Correlation between synovial fluid and serum IL-1beta levels after ACL surgery-preliminary report

Nikica Darabos  1 , Zeljka Hundric-Haspl, Miroslav Haspl, Alemka Markotic, Anela Darabos, Carsten Moser
Affiliations
Randomized Controlled Trial

Correlation between synovial fluid and serum IL-1beta levels after ACL surgery-preliminary report

Nikica Darabos et al. Int Orthop. 2009 Apr.

Abstract
in English, French

The possibility of controlling the harmful intra-articular influence of elevated interleukin (IL)-1beta synovial fluid concentration after anterior cruciate ligament (ACL) surgery could be useful. We investigated the correlation between serum and synovial fluid IL-1beta levels following ACL reconstruction. We measured IL-1beta concentration periodically in three synovial fluid and four serum samples in each of 20 patients receiving either autologous conditioned serum (ACS) containing endogenous anti-inflammatory cytokines including IL-1Ra and several growth factors (group A) or placebo (group B). A decrease in IL-1beta synovial fluid concentration appeared to be more pronounced in absolute terms in group A. In eight patients serum IL-1beta was detected on the 6th postoperative day. In four of them whose synovial fluid levels were over 10 pg/ml on the 6th postoperative day, serum IL-1beta was detected on the 10th postoperative day. The results were different in group B. Correlation between serum and synovial fluid IL-1beta appearance persists in patients after ACL surgery and ACS application. This study is an example of ACS influence on the ACL healing process controlling the IL-1beta levels on the basis of the serum IL-1beta detection.

Le contrôle de l’influence intra-articulaire néfaste de la concentration synoviale en l’IL-1β après chirurgie du croisé antérieur. Matériel et méthode: nous avons analysé les corrélations entre le sérum et le liquide synovial avec dosage des taux de l’IL-1β après la reconstruction du croisé antérieur. Nous avons mesuré la concentration de l’IL-1β de façon périodique avec trois prélèvements synoviaux et 4 dosages sériques chez 20 patients recevant un sérum ACS contenant des cytokines anti-inflammatoires incluant IL-1Ra et les différents facteurs de croissance (Groupe A) ou le Placébo (Groupe B). Résultats: la concentration de l’IL-1β dans le liquide synovial diminue de façon importante dans le groupe A. Chez 8 patients l’IL-1β sérique a été détectée au sixième jour post-opératoire. Pour chacun d’entre-eux le taux d’ IL-1β synovial était supérieur à 10 pg/ml, à la sixième heure post-opératoirel’IL-1β sérique a été détecté au 10ème jour post-opératoire. Les résultats sont très différents dans le groupe B. En conclusion, une corrélation persiste entre les taux sériques et les taux synoviaux de l’IL-1β chez les patients après chirurgie du croisé antérieur. Cette étude est un exemple de l’influence de l’ACS dans le processus de cicatrisation en contrôlant les taux de l’IL-1β et en analysant les taux sériques de celui-ci.

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Figures

Fig. 1
Fig. 1
Synovial fluid IL-1β concentrations measured periodically in three synovial fluid samples
Fig. 2
Fig. 2
Serum IL-1β concentrations measured periodically in four serum samples
See this image and copyright information in PMC

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