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. 1991 Sep 1;174(3):749-52.
doi: 10.1084/jem.174.3.749.

Expression of mRNA and immunoreactivity for the granulocyte/macrophage colony-stimulating factor in activated human eosinophils

R Moqbel  1 , Q Hamid, S Ying, J Barkans, A Hartnell, A Tsicopoulos, A J Wardlaw, A B Kay
Affiliations

Expression of mRNA and immunoreactivity for the granulocyte/macrophage colony-stimulating factor in activated human eosinophils

R Moqbel et al. J Exp Med. .

Abstract

Using in situ hybridization, we have shown that activated human peripheral blood eosinophils express mRNA for granulocyte/macrophage colony-stimulating factor (GM-CSF). Between 15 and 27% of eosinophils gave positive hybridization signals for GM-CSF mRNA after stimulation with the calcium ionophore A23187 or interferon gamma, and 4 and 6% after incubation with interleukin 3 (IL-3) or IL-5. Activated eosinophils also gave specific immunoreactivity with an anti-GM-CSF polyclonal antibody, suggesting translation of the mRNA. These data indicate that eosinophils may be an important source of GM-CSF at sites of allergic inflammation. Furthermore, the identification of GM-CSF production by human eosinophils suggests that the pro-inflammatory potential of this cell type may be substantially greater than hitherto recognized.

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References

    1. J Immunol. 1990 Nov 1;145(9):2950-8 - PubMed
    1. J Exp Med. 1991 Mar 1;173(3):775-8 - PubMed
    1. J Clin Invest. 1991 May;87(5):1541-6 - PubMed
    1. J Exp Med. 1988 Jan 1;167(1):219-24 - PubMed
    1. J Immunol. 1988 Dec 15;141(12):4158-64 - PubMed

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