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. 2008 Aug 27:6:12.
doi: 10.1186/1477-9560-6-12.

Use of rosiglitazone before and after vascular injury in hypercholesterolemic rabbits: Assessment of neointimal formation

Alexandre Alessi  1 , Olímpio Ribeiro França Neto, Paulo Roberto Slud Brofman, Camila Prim, Lucia Noronha, Ruy Fernando Kuenzer Caetano Silva, Liz Andréa Villela Baroncini, Dalton Bertolim Précoma
Affiliations

Use of rosiglitazone before and after vascular injury in hypercholesterolemic rabbits: Assessment of neointimal formation

Alexandre Alessi et al. Thromb J. .

Abstract

Objectives: To analyse the effects of rosiglitazone administered at different times on neointimal formation in hypercholesterolemic rabbits following vascular injury.

Methods: Thirty-nine rabbits on a hypercholesterolemic diet were included. The animals underwent balloon catheter injury to the right iliac artery on day 14. They were divided into three groups as follows: control group, 13 rabbits without rosiglitazone; group I, 13 rabbits treated with rosiglitazone (3 mg/Kg body weight/day) for 28 days after the vascular injury; and group II, 13 rabbits treated with rosiglitazone (3 mg/Kg body weight/day) during all the experiment (42 days). Histological analysis was done by an experienced pathologist who was unaware of the rosiglitazone treatment. Histomorphometric parameters were performed by calculation of the luminal and intimal layer area, and intima/media layer area ratio (the area of the intimal layer divided by the area of the medial layer).

Results: Intimal area was significantly lower in group II vs. CG (p = 0.024) and group I (p = 0.006). Luminal layer area was higher in group II vs. CG (p < 0.0001) and group I (p < 0.0001). Intima/media layer area ratio was equal between CG and group I. Intima/media layer ratio area was significantly lower in group II vs. control group (p < 0.021) and group I (p < 0.003). There was a significant reduction of 65% and 71% in intima/media layer area ratio in group II vs. control group and group I, respectively.

Conclusion: Pretreatment with rosiglitazone in hypercholesterolemic rabbits submitted to vascular injury significantly reduces neointimal formation.

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Figures

Figure 1
Figure 1
Representative histological sections demonstrating neointimal formation – Orcein Staining.Panel A: Control group. Panel B: Group I. Panel C: Group II. NI represents neointima.
Figure 2
Figure 2
Quantification of intima/media layer area ratio; and total intimal layer area.
See this image and copyright information in PMC

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