Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Nov;6(11):1991-3.
doi: 10.1111/j.1538-7836.2008.03147.x. Epub 2008 Aug 28.

Length of tandem repeats in fibrin's alphaC region correlates with fiber extensibility

M R Falvo, D Millard, E T O'Brien 3rd, R Superfine, S T Lord

Length of tandem repeats in fibrin's alphaC region correlates with fiber extensibility

M R Falvo et al. J Thromb Haemost. 2008 Nov.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
(A) Cartoon of fibrin molecule depicting released αC region. The thicker checked portion indicates the approximate location of the tandem repeat segment. (B) Schematics of the α chain for human, mouse and chicken. Solid black indicates the coiled coil region, white indicates the αC region and the repeat segment of the αC is overlaid with a checked pattern. (CE) Fluorescence images of suspended human fibrin fiber stretched to 230% strain with the AFM tip (not visible). Channel/Ridge structures were prepared through microcontact printing using a patterned silicone rubber stamp and a UV curable adhesive to produce 25 micron channels 10 microns deep [3, 4]. Human and mouse fibrinogens, human factor XIII, human and mouse thrombin were purchased from Enzyme Research Laboratories, Inc. Chicken fibrinogen was purified from fresh-frozen chicken plasma. Human thrombin was used to prepare clots from human and chicken fibrinogens; mouse thrombin was used with mouse fibrinogen. Clots prepared under the conditions used for microscopy were analyzed by SDS-PAGE; all showed substantive crosslinking, as identified by gamma-gamma dimers. Final concentration of reagents were 0.02 mg/mL fibrinogen, 0.1 U/mL thrombin, 0.05 μg/mL Factor XIII in 5 mM calcium HBS. Fibrinogen was fluorescently labeled after polymerization with 24nm volume-labeled red fluorescent carboxyl coated microspheres. The fiber is suspended across a channel and manipulated with the AFM tip (not visible). All figures are at same scale; scale bar = 20μm. (G) Histograms of extensibility measurements. (H) Bar graph of averages of the data depicted in the histograms. Error bars represent standard deviations.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Gerth C, Roberts WW, Ferry JD. Rheology of fibrin clots. II. Linear viscoelastic behavior in shear creep. Biophys Chem. 1974;2:208–17. - PubMed
    1. Ryan EA, Mockros LF, Weisel JW, Lorand L. Structural origins of fibrin clot rheology. Biophys J. 1999;77:2813–26. - PMC - PubMed
    1. Liu W, Jawerth LM, Sparks EA, Falvo MR, Hantgan RR, Superfine R, Lord ST, Guthold M. Fibrin fibers have extraordinary extensibility and elasticity. Science. 2006;313:634. - PMC - PubMed
    1. Guthold M, Liu W, Sparks EA, Jawerth LM, Peng L, Falvo M, Superfine R, Hantgan RR, Lord ST. A comparison of the mechanical and structural properties of fibrin fibers with other protein fibers. Cell Biochem Biophys. 2007;49:165–81. - PMC - PubMed
    1. Weisel JW. Fibrinogen and fibrin. Adv Protein Chem. 2005;70:247–99. - PubMed

Publication types