Comparative Study
doi: 10.1038/352689a0.
Cloning of a human gene encoding the general transcription initiation factor IIB
Affiliations
- PMID: 1876184
- DOI: 10.1038/352689a0
Item in Clipboard
Comparative Study
Cloning of a human gene encoding the general transcription initiation factor IIB
Nature.
.
Abstract
Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic sigma-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.
Similar articles
-
Crystal structure of a TFIIB-TBP-TATA-element ternary complex.Nature. 1995 Sep 14;377(6545):119-28. doi: 10.1038/377119a0. Nature. 1995. PMID: 7675079
-
X-ray crystallographic studies of eukaryotic transcription initiation factors.Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):483-9. doi: 10.1098/rstb.1996.0046. Philos Trans R Soc Lond B Biol Sci. 1996. PMID: 8735270 Review.
-
Conserved sequence motifs in the small subunit of human general transcription factor TFIIE.Nature. 1991 Dec 5;354(6352):401-4. doi: 10.1038/354401a0. Nature. 1991. PMID: 1956404
-
Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE.Nature. 1991 Dec 5;354(6352):398-401. doi: 10.1038/354398a0. Nature. 1991. PMID: 1956403
-
Transcriptional activation: enter TFIIB.Trends Biochem Sci. 1991 Sep;16(9):317-8. doi: 10.1016/0968-0004(91)90130-n. Trends Biochem Sci. 1991. PMID: 1949150 Review. No abstract available.
Cited by
-
Single molecule microscopy reveals mechanistic insight into RNA polymerase II preinitiation complex assembly and transcriptional activity.Nucleic Acids Res. 2016 Sep 6;44(15):7132-43. doi: 10.1093/nar/gkw321. Epub 2016 Apr 25. Nucleic Acids Res. 2016. PMID: 27112574 Free PMC article.
-
The Epstein-Barr virus nuclear protein 2 acidic domain forms a complex with a novel cellular coactivator that can interact with TFIIE.Mol Cell Biol. 1995 Sep;15(9):4735-44. doi: 10.1128/MCB.15.9.4735. Mol Cell Biol. 1995. PMID: 7651391 Free PMC article.
-
Photo-cross-linking of a purified preinitiation complex reveals central roles for the RNA polymerase II mobile clamp and TFIIE in initiation mechanisms.Mol Cell Biol. 2004 Feb;24(3):1122-31. doi: 10.1128/MCB.24.3.1122-1131.2004. Mol Cell Biol. 2004. PMID: 14729958 Free PMC article.
-
The nonphosphorylated form of RNA polymerase II preferentially associates with the preinitiation complex.Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10004-8. doi: 10.1073/pnas.88.22.10004. Proc Natl Acad Sci U S A. 1991. PMID: 1946417 Free PMC article.
-
Mapping the sequences that mediate interaction of the equine herpesvirus 1 immediate-early protein and human TFIIB.J Virol. 2001 Nov;75(21):10219-30. doi: 10.1128/JVI.75.21.10219-10230.2001. J Virol. 2001. PMID: 11581390 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
