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. 2008 Sep;42(1):11-21.
doi: 10.1007/s10858-008-9262-2. Epub 2008 Sep 2.

Graphical interpretation of Boolean operators for protein NMR assignments

Dries Verdegem  1 , Klaas Dijkstra, Xavier Hanoulle, Guy Lippens
Affiliations

Graphical interpretation of Boolean operators for protein NMR assignments

Dries Verdegem et al. J Biomol NMR. 2008 Sep.

Abstract

We have developed a graphics based algorithm for semi-automated protein NMR assignments. Using the basic sequential triple resonance assignment strategy, the method is inspired by the Boolean operators as it applies "AND"-, "OR"- and "NOT"-like operations on planes pulled out of the classical three-dimensional spectra to obtain its functionality. The method's strength lies in the continuous graphical presentation of the spectra, allowing both a semi-automatic peaklist construction and sequential assignment. We demonstrate here its general use for the case of a folded protein with a well-dispersed spectrum, but equally for a natively unfolded protein where spectral resolution is minimal.

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References

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