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. 2008 Sep 24;130(38):12636-8.
doi: 10.1021/ja801510d. Epub 2008 Sep 3.

Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety

Minyong Li  1 , Na Lin, Zhen Huang, Lupei Du, Craig Altier, Hao Fang, Binghe Wang
Affiliations

Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety

Minyong Li et al. J Am Chem Soc. .

Abstract

The first general method for the selection of boronic acid-based aptamers (boronolectins) that allows for glycan substructure focusing is described. Using fibrinogen as a model glycoprotein, we have selected boronic acid-modified DNA aptamers that have high affinities (low nM Kd) and the ability to recognize changes in the glycosylation site. The method developed should also be applicable to the development of aptamers for other glycoproducts, such as glycolipids and glycopeptides.

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Figures

Figure 1
Figure 1
The chemical structures of B-TTP and M-TTP
Scheme 1
Scheme 1
SELEX scheme for selecting DNA aptamers with high affinity for fibrinogen
Figure 2
Figure 2
Retention of radioactive DNA on fibrinogen-immobilized beads during 13 rounds of selection
Figure 3
Figure 3
Binding curves of B-TTP-, TTP- and M-TTP-derived 85A with fibrinogen (left), deglycosylated fibrinogen (middle) and periodated fibrinogen (right)
See this image and copyright information in PMC

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