Characterization and expression of a Pinus radiata putative ortholog to the Arabidopsis SHORT-ROOT gene
- PMID: 18765368
- DOI: 10.1093/treephys/28.11.1629
Characterization and expression of a Pinus radiata putative ortholog to the Arabidopsis SHORT-ROOT gene
Abstract
We characterized a Pinus radiata D. Don putative ortholog to the Arabidopsis thaliana (L.) Heynh. SHORT--ROOT gene (AtSHR) and analyzed its expression in different organs during vegetative development and in response to exogenous auxin during adventitious rooting. The predicted protein sequence contained domains characteristic of the GRAS protein family and showed a strong similarity to the SHORT--ROOT (SHR) proteins. Quantitative reverse transcriptase--polymerase chain reaction (qRT-PCR) and in situ hybridization showed that the gene is predominantly expressed in roots, root primordia and in the cambial region of hypocotyl cuttings. Increased mRNA levels were observed, independently of the presence or absence of exogenous auxin, in the cambial region and rooting competent cells of hypocotyl cuttings within the first 24 h of adventitious rooting, before the activation of cell divisions and the organization of the adventitious root meristem. The expression pattern in organs and during adventitious rooting was similar to that of a Pinus radiata SCARECROW-LIKE (PrSCL1) gene, except that PrSCL1 is induced in response to exogenous auxin. Results suggest that the Pinus radiata SHORT-ROOT (PrSHR) gene has a role in root meristem formation and maintenance and in the cambial region of hypocotyl cuttings.
Comment in
-
Reprogramming adult cells during organ regeneration in forest species.Plant Signal Behav. 2009 Aug;4(8):793-5. doi: 10.4161/psb.4.8.9238. Epub 2009 Aug 10. Plant Signal Behav. 2009. PMID: 19820297 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases