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. 2008 Nov;46(11):3636-8.
doi: 10.1128/JCM.01308-08. Epub 2008 Sep 3.

Real-time PCR measurement of persistence of Bordetella pertussis DNA in nasopharyngeal secretions during antibiotic treatment of young children with pertussis

Philippe Bidet  1 , Sandrine Liguori, Agathe De Lauzanne, Valérie Caro, Mathie Lorrot, Agnès Carol, Albert Faye, Nicole Guiso, Edouard Bingen, Stéphane Bonacorsi
Affiliations

Real-time PCR measurement of persistence of Bordetella pertussis DNA in nasopharyngeal secretions during antibiotic treatment of young children with pertussis

Philippe Bidet et al. J Clin Microbiol. 2008 Nov.

Abstract

We used real-time PCR to examine the persistence of Bordetella pertussis DNA in serial nasopharyngeal aspirates from 22 children treated for pertussis. After 5 days of treatment, PCR was positive for all 21 assessable patients. After 14 and 21 days, PCR was still positive for 83% (10/12) and 66% (4/6) of assessable patients, respectively. One patient was tested 1 month after treatment initiation, and B. pertussis DNA was still detectable. Quantitative analysis showed that the DNA concentration diminished during treatment in all except one case. The PCR cycle threshold at which B. pertussis DNA became detectable increased by a mean of 1.7 cycles per day (range, 0.86 to 3.68 cycles per day). Real-time PCR can thus be used to diagnose pertussis in young children for up to 3 weeks after treatment initiation. Its potential value for assessing the treatment outcome remains to be determined.

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Figures

FIG. 1.
FIG. 1.
Time course of IS481 real-time PCR CT values applied to serial nasopharyngeal samples from 18 infants treated for pertussis.
See this image and copyright information in PMC

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