doi: 10.1002/anie.200800736.
An engineered protease that cleaves specifically after sulfated tyrosine
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- PMID: 18780393
- PMCID: PMC2671331
- DOI: 10.1002/anie.200800736
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An engineered protease that cleaves specifically after sulfated tyrosine
Angew Chem Int Ed Engl.
2008.
No abstract available
Figures
a) Two-color flow-cytometric scheme for the isolation of sulfotyrosine-specific OmpT variants. BD =BODIPY, TMR =tetramethylrhodamine, Q =QSY 7. b) Fluorescence profiles (emission at λ =530 nm with a 30 nm filter) of cells expressing no enzyme (BL21(DE3), red trace), sT1 (blue), sT2 (purple), sT3 (cyan), and sT4 (yellow) labeled with the sTyr↓Arg screening substrate 1 a. c) Sequences of the substrates used for screening and characterization of the OmpT variants.
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