High-performance liquid chromatographic analysis of 5-ethylpyrimidines and 5-methylpyrimidines in plasma
- PMID: 1878072
- DOI: 10.1016/0378-4347(91)80047-g
High-performance liquid chromatographic analysis of 5-ethylpyrimidines and 5-methylpyrimidines in plasma
Erratum in
- J Chromatogr 1991 Apr 19;565(1-2):552
Abstract
A high-performance liquid chromatographic (HPLC) method employing a C18 reversed-phase column, a mobile phase of sodium acetate and methanol, and an ultraviolet detector was developed for the analysis of 5-ethylpyrimidines and 5-methylpyrimidines in plasma. Samples were prepared for HPLC by sequential cation-exchange and anion-exchange column chromatography. Linear standard curves were obtained for samples containing 0.05-50 micrograms/ml 5-ethyl-2'-deoxyuridine and 5-ethyluracil, 0.05-10 micrograms ml 5-(1-hydroxyethyl)uracil, and 0.1-50 micrograms/ml thymidine, thymine and 5-hydroxymethyluracil. Applicability of the method to determination of the kinetics of 5-ethyl-2'-deoxyuridine elimination by the isolated perfused rat liver was demonstrated; clearance of the drug was 1.29 ml/min.
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