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. 2008 Nov;46(11):3798-9.
doi: 10.1128/JCM.01551-08. Epub 2008 Sep 10.

Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay

J L Guthrie  1 , C Seah, S Brown, P Tang, F Jamieson, S J Drews
Affiliations

Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay

J L Guthrie et al. J Clin Microbiol. 2008 Nov.

Abstract

This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

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Figures

FIG. 1.
FIG. 1.
Analytical sensitivity of BP3385 PCR in comparison to IS481 PCR, using cell suspensions of B. pertussis ATCC 9797 in a real-time PCR assay.
See this image and copyright information in PMC

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