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. 2008 Sep 14;14(34):5327-30.
doi: 10.3748/wjg.14.5327.

Anti-HBc screening in Indian blood donors: still an unresolved issue

Hari-Krishan Dhawan  1 , Neelam Marwaha, Ratti-Ram Sharma, Yogesh Chawla, Beenu Thakral, Karan Saluja, Sanjeev-Kumar Sharma, Manish-K Thakur, Ashish Jain
Affiliations

Anti-HBc screening in Indian blood donors: still an unresolved issue

Hari-Krishan Dhawan et al. World J Gastroenterol. .

Abstract

Aim: To study the seroprevalence of antibody to hepatitis B core antigen (anti-HBc) in healthy blood donors negative for HBsAg and to evaluate whether anti-HBc detection could be adopted in India as a screening assay for HBV in addition to HBsAg.

Methods: A total of 1700 serum samples collected from HBsAg-negative healthy blood donors were tested for the presence of anti-HBc antibody (IgM + IgG). All samples reactive for anti-HBc antibody were then investigated for presence of anti-HBs and for liver function tests (LFTs). One hundred serum samples reactive for anti-HBc were tested for HBV DNA by PCR method.

Results: Out of 1700 samples tested, 142 (8.4%) blood samples were found to be reactive for anti-HBc. It was significantly lower in voluntary (6.9%) as compared to replacement donors (10.4%, P = 0.011). Seventy-two (50.7%) anti-HBc reactive samples were also reactive for anti-HBs with levels > 10 mIU/mL and 70 (49.3%) samples were non-reactive for anti-HBs, these units were labeled as anti-HBc-only. These 142 anti-HBc reactive units were also tested for liver function test. HBV DNA was detected in only 1 of 100 samples tested.

Conclusion: Keeping in view that 8%-18% of donor population in India is anti-HBc reactive, inclusion of anti-HBc testing will lead to high discard rate. Anti-HBs as proposed previously does not seem to predict clearance of the virus. Cost effectiveness of introducing universal anti-HBc screening and discarding large number of blood units versus considering ID NAT (Individual donor nucleic acid testing) needs to be assessed.

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