doi: 10.1016/j.mimet.2008.08.006.
Epub 2008 Aug 26.
Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments
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- PMID: 18789360
- DOI: 10.1016/j.mimet.2008.08.006
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Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments
J Microbiol Methods.
2008 Dec.
Abstract
Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by approximately 23%.
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