Strong coupling between pyramidal cell activity and network oscillations in the olfactory cortex

Abstract

Oscillatory activity is a prominent characteristic of electrophysiological recordings in the olfactory system and has been proposed to play a key role in encoding olfactory representations. Studies in several systems have shown that some aspects of information coding involve characteristics that intertwine spikes and fast oscillations (in the beta and gamma range) of local field potentials (LFP). In the insect olfactory system, it has been proposed that oscillatory activity could provide a temporal link between cells. Following previous data, we have proposed that gamma band oscillations in mammals could subserve a gating function for the transfer of information between the olfactory bulb (OB) and the anterior piriform cortex (aPC), which are functionally coupled. In this study, we used an electrophysiological approach to investigate the temporal relationship between LFP gamma oscillations and single-unit activity by simultaneously recording LFP and single unit discharges in the rat aPC during odor evoked activity. Our data showed that mean spike discharges and gamma oscillatory bursts were synchronized with the same respiratory cycle epoch (around the inspiration/expiration transition). Temporal correlations between spikes and LFP revealed that cortical cell spikes were tightly phase-coupled with the peak of gamma oscillations and that this phase-coupling was not odor-dependent. Our results suggest that gamma oscillation may act as a temporal filter. Oscillatory phase-coupled spikes in the OB could act in increasing the probability of spike emission in the aPC cell during a narrow time-window, explaining the tight phase-coupling observed in the aPC. The role of spike-LFP phase-coupling as a binding function between odor features is discussed.