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Comparative Study
. 2008 Nov;52(11):4145-8.
doi: 10.1128/AAC.00959-08. Epub 2008 Sep 15.

Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans

Nathan P Wiederhold  1 , Jodi L Grabinski, Guillermo Garcia-Effron, David S Perlin, Samuel A Lee
Affiliations
Comparative Study

Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans

Nathan P Wiederhold et al. Antimicrob Agents Chemother. 2008 Nov.

Abstract

Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.

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Figures

FIG. 1.
FIG. 1.
FKS1 sequence results for C. albicans for the two isolates not in full agreement, 5415 (A) and 42286 (B and C), as determined by pyrosequencing (A and C) and by Sanger dideoxy sequencing (C).
See this image and copyright information in PMC

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