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. 1991 Jul 15;103(1):97-9.
doi: 10.1016/0378-1119(91)90398-u.

A thiostrepton-inducible expression vector for use in Streptomyces spp

S Kuhstoss  1 , R N Rao
Affiliations

A thiostrepton-inducible expression vector for use in Streptomyces spp

S Kuhstoss et al. Gene. .

Abstract

A shuttle expression vector containing the thiostrepton-inducible Streptomyces lividans promoter, ptipA, and the origin of transfer from plasmid RP4 was constructed. Cassettes containing a promoterless xylE gene upstream from a hyg gene were used to demonstrate thiostrepton-inducible expression from ptipA in both S. lividans and Streptomyces ambofaciens, ptipA was estimated to be induced 60-fold or more in Streptomyces ambofaciens.

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