Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2008 Nov;46(11):3672-7.
doi: 10.1128/JCM.00913-08. Epub 2008 Sep 17.

Kinetics of Epstein-Barr virus DNA load in different blood compartments of pediatric recipients of T-cell-depleted HLA-haploidentical stem cell transplantation

Fausto Baldanti  1 , Marta Gatti, Milena Furione, Stefania Paolucci, Carmine Tinelli, Patrizia Comoli, Pietro Merli, Franco Locatelli
Affiliations
Comparative Study

Kinetics of Epstein-Barr virus DNA load in different blood compartments of pediatric recipients of T-cell-depleted HLA-haploidentical stem cell transplantation

Fausto Baldanti et al. J Clin Microbiol. 2008 Nov.

Abstract

Epstein-Barr virus (EBV) DNA levels in whole-blood samples of 54 pediatric patients receiving T-cell-depleted haploidentical hematopoietic stem cell transplantation (HSCT) in 2003 to 2007 were retrospectively compared with EBV DNA loads in peripheral blood mononuclear cells (PBMC). Determination of EBV DNA in whole blood missed 1 of 19 patients (5.2%), who tested positive for EBV DNA in PBMC. The analytical sensitivity of EBV DNA detection in whole-blood samples relative to that in PBMC was 94.7%. Regression analysis showed a significant correlation between DNA levels in PBMC and whole blood (r = 0.81; P < 0.001). Relative to that in PBMC, the appearance of EBV DNA in whole blood was delayed in 9/18 patients (median, 49 days; range, 6 to 226 days), while peak levels and clearance were reached simultaneously. Following peak levels, EBV DNA showed a slower decline in whole blood than in PBMC. In conclusion, (i) EBV DNA levels in PBMC were significantly correlated with those in whole blood; (ii) a differential kinetics of EBV DNA in the two blood compartments was observed; and (iii) monitoring of EBV DNA levels in whole blood appears to be a valuable alternative to PBMC in the follow-up of pediatric recipients of haploidentical T-cell-depleted HSCT.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Regression analysis of EBV DNA levels in PBMC and whole blood of pediatric haploidentical HSCT recipients.
FIG. 2.
FIG. 2.
Kinetics of EBV DNA in PBMC and whole-blood samples of pediatric haploidentical HSCT recipients. (A) Percentages of patients (pts) positive for EBV DNA in PBMC (filled bars) and in whole blood (shaded bars) with respect to the time of peak levels in PBMC. (B) Mean log10 copies of EBV DNA in PBMC (filled bars) and in whole blood (shaded bars) with respect to the time of peak levels in PBMC. (C) EBV DNA levels expressed as mean percentages of peak EBV DNA levels in PBMC (filled bars) and in whole blood (shaded bars) with respect to the time of peak levels in PBMC.
FIG. 3.
FIG. 3.
Representative EBV DNA kinetics in PBMC (filled circles) and whole-blood (open circles) samples of six pediatric haploidentical HSCT recipients. EBV DNA levels in PBMC are expressed as the number of EBV DNA copies per 1 × 105 cells, while EBV DNA levels in whole blood are expressed as the number of EBV DNA copies per milliliter. pt, patient.
See this image and copyright information in PMC

References

    1. Aalto, S. M., E. Juvonen, J. Tarkkanen, L. Volin, H. Haario, T. Ruutu, and K. Hedman. 2007. Epstein-Barr viral load and disease prediction in a large cohort of allogeneic stem cell transplant recipients. Clin. Infect. Dis. 451305-1309. - PubMed
    1. Bakker, N. A., G. W. van Imhoff, E. A. M. Verschuuren, and W. J. van Son. 2007. Presentation and early detection of post-transplant lymphoproliferative disorder after solid organ transplantation. Transpl. Int. 20207-218. - PubMed
    1. Bakker, N. A., E. A. Verschuuren, N. J. Veeger, W. van der Bij, G. W. van Imhoff, C. G. Kallenberg, and B. G. Hepkema. 2008. Quantification of Epstein-Barr virus-DNA load in lung transplant recipients: a comparison of plasma versus whole blood. J. Heart Lung Transplant. 277-10. - PubMed
    1. Baldanti, F., P. Grossi, M. Furione, L. Simoncini, A. Sarasini, P. Comoli, R. Maccario, R. Fiocchi, and G. Gerna. 2000. High levels of Epstein-Barr virus DNA in blood of solid-organ transplant recipients and their value in predicting posttransplant lymphoproliferative disorders. J. Clin. Microbiol. 38613-619. - PMC - PubMed
    1. Capello, D., D. Rossi, and G. Gaidano. 2005. Post-transplant lymphoproliferative disorders: molecular basis of disease histogenesis and pathogenesis. Hematol. Oncol. 2361-67. - PubMed

Publication types