Review
doi: 10.1016/j.bbamem.2008.08.013.
Epub 2008 Aug 29.
Fluorescence correlation spectroscopy in membrane structure elucidation
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- PMID: 18801332
- DOI: 10.1016/j.bbamem.2008.08.013
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Review
Fluorescence correlation spectroscopy in membrane structure elucidation
Biochim Biophys Acta.
2009 Jan.
Free article
Abstract
This review describes the application of fluorescence correlation spectroscopy (FCS) for the study of biological membranes. Monitoring the fluorescence signal fluctuations, it is possible to obtain diffusion constants and concentrations for several membrane components. Focusing the attention on lipid bilayers, we explain the technical difficulties and the new FCS-based methodologies introduced to overcome them. Finally, we report several examples of studies which apply FCS on both model and biological membranes to obtain interesting insight in the topic of lateral membrane organization.
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