Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Nov;3(11):3173-92.
doi: 10.1093/nar/3.11.3173.

Stability of nucleosomes in native and reconstituted chromatins

J E Germond, M Bellard, P Oudet, P Chambon
Free PMC article

Stability of nucleosomes in native and reconstituted chromatins

J E Germond et al. Nucleic Acids Res. 1976 Nov.
Free PMC article

Abstract

The stability of nucleosomes of SV40 minichromosomes extracted from infected cells or reconstituted by association of SV40 DNA and the four histones H2A, H2B, H3 and H4 was studied as a function of the ionic strength. As a measure of the stability of the nucleosome, we followed the disappearance of the nucleosomes from the original chromatin and their appearance on a "competing" DNA. We show here that the DNA and the histone components of the nucleosomes do not apprecially dissociate below 800 mM NaCl. At 800 mM and above, the histone moiety of the nucleosomes can dissociate from the DNA and efficiently participate to the formation of nucleosomes on a "competing" DNA.

PubMed Disclaimer

References

    1. Cell. 1975 Apr;4(4):281-300 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 May;72(5):1843-7 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:199-208 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1092-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2626-30 - PubMed